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Bradford Reagent Disposal

Bradford Reagent Disposal



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I am a graduate student volunteering in a professor's lab being tasked with finding out how to dispose of certain hazardous materials. I have encountered a problem with disposing of Bradford's Reagent. I have checked online, but am running into problems due to the methanol component of this compound. Can someone help me with the proper disposal procedure? Thank you in advance.


I would strongly suggest to ask someone in your lab about this, they will have a better idea about the different waste disposal methods you have available.

In general you would dispose anything that contains organic solvents like methanol in a waste container for generic solvent waste. You should have something like that somewhere in the lab.

One thing you always have to keep in mind is to never put anything still reactive into your waste container. A popular example would be a strong oxidizing agent, putting that into a solvent waste container is dangerous and could e.g. ignite the waste. This is not an issue in this case, but you should always keep that in mind.

Another aspect is the pH of the waste, in many cases the waste disposal facility will only accept reasonably neutral waste, so you should neutralize your waste before putting it into a container. Neutralizing it inside the container can be much more annoying. This might not be necessary if you have a dedicated acidic waste, you'll have to ask someone in your lab about that. The Bradford reagent is strongly acidic, so you'll have to pay attention to this aspect.


Evaluation of Colorimetric Assays for Analyzing Reductively Methylated Proteins: Biases and Mechanistic Insights

Colorimetric protein assays, such as the Coomassie blue G-250 dye-binding (Bradford) and bicinchoninic acid (BCA) assays, are commonly used to quantify protein concentration. The accuracy of these assays depends on the amino acid composition. Because of the extensive use of reductive methylation in the study of proteins and the importance of biological methylation, it is necessary to evaluate the impact of lysyl methylation on the Bradford and BCA assays. Unmodified and reductively methylated proteins were analyzed using the absorbance at 280 nm to standardize the concentrations. Using model compounds, we demonstrate that the dimethylation of lysyl ε-amines does not affect the proteins’ molar extinction coefficients at 280 nm. For the Bradford assay, the response (absorbance per unit concentration) of the unmodified and reductively methylated proteins were similar with a slight decrease in the response upon methylation. For the BCA assay, the responses of the reductively methylated proteins were consistently higher, overestimating the concentrations of the methylated proteins. The enhanced color-formation in the BCA assay may be due to the lower acid dissociation constants of the lysyl ε-dimethylamines, compared to the unmodified ε-amine, favoring Cu(II) binding in biuret-like complexes. The implications for the analysis of biologically methylated samples are discussed.


J61522 Bradford Dye Reagent, Ready-to-Use soln.

The Bradford Reagent is suitable for protein concentration determination (100-1500µg).Bradford dye reagent is used in the determination of protein content in cell fractions and assessing the protein concentrations.

Примечания

Ссылки на литературу

Jorge-Araújo, P. Quiquampoix, H. Matumoto-Pintro, P. T. Staunton, S. Glomalin-related soil protein in French temperate forest soils: interference in the Bradford assay caused by co-extracted humic substances. Eur. J. Soil Sci. 2015, 66 (2), 311-319.

Aminian, M. Nabatchian, F. Vaisi-Raygani, A. Torabi, M. Mechanism of Coomassie Brilliant Blue G-250 binding to cetyltrimethylammonium bromide: An interference with the Bradford assay. Anal. Biochem. 2015, 434 (2), 287-291.

Фразы опасности и предосторожности СГС

Фразы опасности (ЕС): H290-H302+H332-H314-H371

May be corrosive to metals. Harmful if swallowed. Harmful if inhaled. Causes severe skin burns and eye damage. May cause damage to organs.

Фразы предосторожности: P264b-P270-P271-P280-P304+P340-P305+P351+P338-P310-P330-P331-P363-P390-P402-P403+P233-P406b-P501c

Wash face, hands and any exposed skin thoroughly after handling Do not eat, drink or smoke when using this product. Use only outdoors or in a well-ventilated area. Wear protective gloves/protective clothing/eye protection/face protection. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER or doctor/physician. Rinse mouth. Do NOT induce vomiting. Wash contaminated clothing before reuse. Absorb spillage to prevent material damage. Store in a dry place. Store in a well-ventilated place. Keep container tightly closed. Store in corrosive resistant polypropylene container with a resistant inliner Dispose of contents/ container to an approved waste disposal plant


Bioreagents

BIOVECTRA offers a selection of high-quality Bioprocessing Reagents that are available in a variety of sizes from gram quantities for research and testing to multi-kg scale for manufacturing operations. Several of our Bioprocessing Reagents are available in cGMP grade, which provides our customers with an industry-leading level of quality and compliance to support their cGMP manufacturing operations.

BIOVECTRA also offers Diagnostic & Molecular Biology Reagents. These products are traditionally used as substrates for the detection of diagnostic enzymes or in molecular biology.

Learn More about Our Facilities
Product Description / Use CAS # BIOVECTRA Catalogue #
DTT Dithiothreitol Industry-leading quality and reliability (Purity ≥ 99%)
Reagent used for maintaining thiol groups in a reduced state during protein processing, isolation, and denaturing
Incorporated into some leading COVID-19 testing kits
3483-12-3 1365
Dithiothreitol – Molecular Biology Grade Premium MBG grade
Includes additional testing for: Protease, Rnase, Dnase, ICP metals
3483-12-3 1365 MBG
Dithiothreitol – cGMP Premium cGMP grade
Includes additional testing: Residual solvents
3483-12-3 1370
DTE Dithioerythritol Reagent used for maintaining thiol groups in a reduced state during protein processing, isolation, and denaturing
Purity ≥ 99%
6892-68-8 1360
DHAA Dehydroascorbic acid
– cGMP
Premium cGMP grade
Reagent used in the oxidation of proteins and prep of ADC’s
490-83-5 1310
TCEP Tris (2-carboxyethyl) phosphine, hydrochloride – cGMP Premium cGMP grade
Reagent used in the reduction of disulfide bonds and during prep of ADC’s
51805-45-9 1301
IPTG Isopropyl-β-D-Thiogalactopyranoside,
Non-Animal Origin
Galactosidase activation reagent used in recombinant microbial fermentation processes
Made with non-animal derived materials
367-93-1 1900
EDAC 1-(3-Dimethylaminopropyl)-3-Ethylcarbodiimide HCI Water soluble cross-linker used to conjugate proteins and peptides 25952-53-8 1339
PEP-K Phosphoenolpyruvate, Monopotassium Salt A substrate for the detection of Carboxylase 4265-07-0 2553
Phosphoenolpyruvate, Tricyclohexylammonium Salt Substrate for the detection of Carboxylase 35556-70-8 2555
p-Nitrophenylphosphate, Ditris Salt Substrate for the detection of Alkaline Phosphatase 68189-42-4 2372
BCIP-T 5-Bromo-4-Chloro-3-Indolyl Phosphate, p-Toluidine Salt Substrate for the detection of alkaline phosphatase, frequently in immunodiagnostic applications 6578-06-9 1176
XTT Benzene Sulfonic Acid Hydrate Widely used to measure cell proliferation in toxicity assays 111072-31-2 2525
MTX Methotrexate, USP Reagent used as a cell selection reagent in the production and maintenance of high producing mammalian cell lines 59-05-2 1322
IPTG Isopropyl-β-D-Thiogalactopyranoside,
Non-Animal Origin
Galactosidase activation reagent used in recombinant microbial fermentation processes
Made with non-animal derived materials
367-93-1 1900
EDAC 1-(3-Dimethylaminopropyl)-3-Ethylcarbodiimide HCI Water soluble cross-linker used to conjugate proteins and peptides 25952-53-8 1339

Loading and Unloading the Autoclave Safely

Contaminated materials should never be left in hallways or other public spaces prior to autoclaving. Biohazard bags should remain in the laboratory until they are ready to be placed in the autoclave. Never leave bags sitting on the floor next to the autoclave.. Bags that are closed and ready for autoclaving must be placed in secondary containment as shown. If the bags are being transported to the autoclave, they must be contained in closed, hardwalled secondary containers.

Minimize contact with biohazard waste as much as possible. Never crush or push down biohazard waste, Biohazard waste containers should be removed for autoclaving when they are 2/3 full. Indicator tape should be applied when placing the new autoclave bag into the hard-walled outer container this will reduce handling of the biohazard waste during removal. The heat sensitive autoclave tape should be placed in an “X” pattern over the biohazard symbol. The heat sensitive tape is to be of the type that changes color, such as the type that stripes appear on after treatment. This tape is available from Fisher Scientific as # 15-903. Once the autoclave disinfection is complete, the tops of the bags may be sealed tightly with lab tape.

After the proper autoclave waste decontamination steps are followed as listed below, the decontaminated waste is then placed in a 44 gallon or 32 gallon white Rubbermaid Brute container (with a drum dolly), lined with black plastic garbage bags, and located in the vicinity of the autoclave. These containers are to be labeled “AUTOCLAVED/ DECONTAMINATED WASTE ONLY” (labels). Biohazard bags placed in the white Brute containers and marked with the heat sensitive tape signal to Housekeeping that the waste is safe and ready to be removed from the laboratory for disposal in the dumpster.

Each department is responsible for providing an adequate number of these containers which are available from the Fisher Scientific. Housekeeping will not remove or otherwise handle overflowing waste or waste in untreated biohazard bags.


LIAISON® XL Main Features

Assay technology

LIAISON ® XL is a fully automated chemiluminescence analyzer, performing the complete sample processing. LIAISON ® XL adopts a “Flash” chemiluminescence technology (CLIA) with paramagnetic microparticle solid phase. The same technology is also used on the LIAISON ® analyzer offering a perfect compatibility between the systems.

Reagents Area

The reagents area is temperature controlled. The loading bay for reagents allows continuous loading of the LIAISON ® XL system of Integrals or Ancillary reagent. For clarity, an LED below each lane indicates the relevant usage status. Up to 25 integrals and up to 4 ancillary reagents can be loaded at the same time. Integrals can be loaded directly in one of the 25 lanes according to the specifications of the LIAISON ® XL, multiple of the same Integral can be present in the reagent area at the same time if needed.
Reagent Integrals are managed with a “Quick Release” feature and are released by the system as soon as the requested process is completed. Starter reagents, responsible for initiating the chemiluminescent reaction, are available on board in a dedicated area and continuosly monitored (lot number, quantity available) and allow continuous loading.

Reagents integral

The reagent Integral contains all assay specific reagents required to perform the assay. All reagents are ready-to-use and includes the calibrator into the Integral however there are few exceptions with calibrators supplied as lyophilized component (but in the Integral box) and the HCV assay that require an Ancillary reagent. All the reagents are identified to and monitored by the system. All information concerning number of tests, lot number, stability on board and/ or calibration validity are automatically updated onto the system. Due to the temperature control of the reagent area, most reagent Integrals have a long on board stability.

Disposable sample tips/cuvettes

No sample carry over is observed on the LIAISON ® XL due to adoption of single-use sample tips and single use reaction module (cuvettes). The quantity of cuvettes and sample tips on board is monitored by the instrument software. Due to the large storage of disposables on board, the LIAISON ® XL permits a extended walk away time.

Wash solution and waste

LIAISON ® XL has a designated area for wash solutions and waste materials (solid and liquid). All containors can accessed and removed throughout use of the instrument.

Sample area

The samples are loaded using 10 racks, each allowing space for 12 samples for a total of 120 samples in continuous loading. Different sized sample tubes can be loaded according with LIAISON ® XL software indications.
All racks and samples are identified via a bar code reader and any identification problems (mis-read, etc) are reported to the user.

The “quick release” feature allows the sample in the sampling process rack to be released by the system as soon as it is completed.

The quality of all samples are monitored, any sample containing clots or with insufficient volume will be flagged.

Software

LIAISON ® XL is easy to use with functional icons that help the operator. All the activities planned and scheduled are continuously monitored and all the results, sample and calibrators and quality controls are traced. The software informs the operator about the status of the systems (Stand by Ready Run) and gives the user an estimation of the consumables needed to perform the number of the tests requested. The operator is allowed to increase the consumable volume before starting the instrument. On every screen-page there is the Help feature, that offers the operator an online operator guide. The LIAISON ® XL is connectable to LIS (Laboratory Information System) and offers a remote service access with direct access from our central service to the system.

LIAISON® XL performance

The throughput is optimized by splitting the pipetting phases of reagents and samples into different cycles. The innovative technical solution used on the LIAISON ® XL, allows double washing capacity and allows the incubator to host up to 80 cuvettes. The optimization of the assay process maintains the throughput of the system as high as possible.


The only substances allowed down drains are those that meet all of the following criteria:

  1. Nonhazardous
    • No radioactive waste
    • No hazardous chemical waste
    • No untreated biohazardous waste
  2. Liquid
    • No solids, sludges, or viscous substances
  3. Will not interfere with sewage treatment operations
    • No corrosive pH levels
    • No grease or oil
    • No hot (150°F or higher) temperatures in volumes of more than 10 gallons

Click on a category below to find out if a substance is allowed to go down the drain.

  • If you can't find a particular substance and aren't sure how to dispose of it, contact the EH&S Environmental Management Facility, (858) 534-3660.

Note: Protect drains from chemical spills — do not use sinks for chemical storage or secondary containment. Disposal of hazardous waste using sinks, intentional evaporation, or as regular trash is against the law. Campus laboratories must abide by strict state and federal waste disposal requirements. You may be held liable for violations of applicable laws.

  • Deactivate with bleach (1 part bleach to 9 parts liquid waste) for 30 minutes before pouring it down the drain.
  • Occasionally, liquid waste is autoclaved. Do not add bleach in this case. Read Autoclaving Guidelines for Biohazardous Waste.

For chemical disinfection of liquid biohazardous waste, the only university-wide approved disinfectant for UC San Diego is bleach. If your laboratory wishes to inquire about the use of alternative disinfectants to inactivate liquid biohazardous waste, please   send an email   with the following information:

  • Material to be disinfected
  • Chemical to be used
  • Concentration of chemical
  • Contact time
  • Disposal method (sewering, hazardous waste pickup)

You will receive a response within five (5) working days regarding approval.

For more information and for disposal instructions for solid biohazardous waste, read How to Package and Dispose of Biohazardous and Medical Waste .


RESEARCH

Basic biological research has evolved into a broad, fast-paced and dynamic profession that drives newly emerging industries and actively shapes many human endeavors. Both globally and locally, cutting-edge research at Texas A&M University strives to understand the fundamental processes driving life around us and to improve the ways people everywhere manage their health and the health of our planet. The Department of Biology is responsible for research and teaching within the vast disciplines of the biological sciences, from molecular cell biology to ecology and evolutionary biology. Our faculty perform cutting-edge research in a wide array of biological sciences in the laboratory and in the field.


Ready-to-use Buffers and Reagents

In addition to our catalogue, Boston BioProducts offers custom services through our experienced team of scientists and product development specialists.

Sample Request

Try a free product from our catalogue before the start of your next research project.

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Novartis Institutes for Biomedical Research

High quality products with a fair price.

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Brigham and women’s hospital

Reliable buffers, very fast turnaround and very good customer service.

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Silicon Therapeutics

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Bradford Protein Quantification kit

The protein quantification method using Coomassie Brilliant Blue G-250 dye has been described for the first time in 1975. Three charge forms of the Coomassie Brilliant Blue dye are present in equilibrium at the usual acidic pH of the assay. The red, bl.

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Bradford, M. M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248&ndash254 (1976)

Chial HJ, Thompson HB, Splittgerber AG. A spectral study of the charge fo

Cross selling for this product : Bradford Protein Quantification kit There are 4 products.

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Watch the video: Bradford Assay (August 2022).