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As we have seen, glycolysis consists in the transformation of one glucose molecule over several steps into two pyruvic acid molecules.

In this process four hydrogens are released, which combine two by two, with molecules of a cell substance capable of receiving them: NAD (nicotinamide adenine dinucleotide).

Upon receiving the hydrogens, each NAD molecule becomes NADH2. During the process, sufficient energy is released for the synthesis of 2 ATP.