Can a gene be expressed under the T7 promoter in an E. coli strain (e.g. DH5 alpha), which does not have the T7 polymerase gene encoded in its genome? In other words, is T7 promoter leaky? To be…
It is well known that water scarcity in the northeastern semi-arid is a problem that requires a priority response. Its cause is low rainfall and irregular rainfall, and a geological structure that does not allow satisfactory accumulation of water in the subsoil, which even interferes in the river regime.
Say I have a species of bacteria. I want this species to only persist for a set number of generations, after which it simply stops reproducing. Is it possible to do this at all (with known genetic
I am searching for source, that providing information about enzymesproteins, in different types of cancers, that their amount in cell is significantly higher - comparing to normal, healthy cell. …
While researching the F12 cell culture medium (Ham, 1965), I came across the term "essentially diploid Chinese hamster ovary cells". The terms "subdiploid" and "near diploid" were also used to refe…
A. What is biochemistry and who needs it?Literally & 34;Biochemistry& 34; is the chemistry of life. This is synonymous with & 34;Molecular Biology& 34;, but traditionally biochemistry has dealt more with the study of proteins and smaller biomolecules and molecular biology has dealt more with gene expression and associated molecules.
